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KMID : 0613820100200101544
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Journal of Life Science
2010 Volume.20 No. 10 p.1544 ~ p.1548
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Relationship between Moxifloxacin Resistance Pheno- and Genotype of Moxifloxacin-Resistant Mycoplasma hominis Obtained in vitro
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Park In-Dal
Choi Myung-Won
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Abstract
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Moxifloxacin (MF) - resistant mutants of Mycoplasma hominis (M. hominis) were generated by stepwise selection in increasing concentrations of MF, and six strains of MF resistant M. hominis mutants - M1, M4, M8, M16, M32, and M64 - in which MICs of MF were 0.5, 4, 8, 16, 32, 64 ㎍/㎖, respectively, were generated. Compared to the sequence of M. hominis PG21, all mutants harbored amino acid substitutions of Arg-163 Thr in GyrA, and Pro-445 Gln in ParE. While the concentrations were getting higher, an additional amino acid substitution was found at Ser-153 Lys in GyrA (≥4 ㎍/㎖), Ser-91 Ile in ParC (≥16 ㎍/㎖), and Val-450 Phe (≥64 ㎍/㎖) in GyrB. These substitutions seem to have an impact on resistance to MF, and GyrB change was found only in the highest concentration and seems to be associated with high-level resistance to MF. This, as far as we know, is the first description of a relationship between MF resistance phenotype and genotype.
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KEYWORD
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Mycoplasma hominis, moxifloxacin, resistance, QRDRs, mutations
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